Methods for Diagnosing Diseases of the Uterine Cervix

Abstract

 Goal: was - the study of the state of the vaginal microbiocenosis and determination of the presence of diseases of the cervix, using the PCR method (Femoflor-16) - for the differential diagnosis of dysbiotic disorders and infectious and inflammatory diseases of genitalia in women.

Methods: Clinical and laboratory examination of 105 patients with cervical diseases was carried out. All patients underwent the necessary diagnostic standard: examination of the cervix in the mirrors, cytological examination of smears from the ecto- and endocervix, polymerase chain reaction (PCR) for HPV, pH-metry, extended colposcopy, histological examination of cervical biopsies. Material for the study of vaginal microbiocenosis was collected from the posterolateral wall of the vagina, for HPV detection - from the cervical canal. The scraping was placed in an Eppendorf tube containing 1 ml of saline; storage and transportation of the material was carried out in accordance with the current regulatory documents. DNK was isolated using a PROBA-GS reagent kit (Standard Diagnostics, Bukhara). The study was carried out by PCR with detection of results in real time (RT-PCR) using Femoflor-16 reagents (Standard Diagnostics, Bukhara) in a detecting amplifier DT-96, according to the manufacturer's instructions, in the laboratory (Standard Diagnostics, g . Bukhara).

Results: In our study, in women with complaints of minor and moderate vaginal discharge - 41.7%, severe vaginal discharge disturbed 7.8% of patients. Complaints of itching and or burning were reported by 33% of patients, vaginal discomfort - 30%, urination disorder - 15.5%, pain during intercourse - 11.6%.

Conclusions: The variety of leading pathogens dictates the need for a comprehensive study of the vaginal microbiocenosis in patients with precancerous pathology of the cervix, which allows, with high accuracy and specificity, to give a quantitative and qualitative assessment of the main participants in the studied biotope for the purpose of individual therapy.